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›› 2010, Vol. 10 ›› Issue (3): 588-592.

• 生化工程专栏 • Previous Articles     Next Articles

Comparison of 2,3-Butanediol Production by Two Klebsiella pneumoniae Strains

SUN Li-hui DAI Jian-ying WANG Xu-dong XIU Zhi-long   

  1. Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology School of Life Science and Biotechnology, Dalian University of Technology Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology
  • Received:2010-02-25 Revised:2010-05-05 Online:2010-06-20 Published:2010-06-20
  • Contact: XIU Zhi-long

两株Klebsiella pneumoniae菌发酵生产2,3-丁二醇的比较

孙丽慧 戴建英 王旭东 修志龙   

  1. 大连理工大学生命科学与技术学院 大连理工大学生命科学与技术学院 大连理工大学环境与生命学院生物科学与工程系 大连理工大学环境与生命学院生物科学与工程系
  • 通讯作者: 修志龙

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Abstract: The fed-batch fermentation for 2,3-butanediol production by Klebsiella pneumoniae DSM 2026 and CICC 10011 strains was investigated. The results showed that CICC 10011 produced less organic acids and ethanol, but more target products and more yield could be obtained than those of DSM 2026. The final concentration of target products (2,3-BD and acetoin) was 85.61 g/L with a productivity of 1.53 g/(L×h) for 56 h, corresponding to 45% of yield from glucose. Comparison of the metabolic flux distributions between K. pneumoniae CICC 10011 and DSM 2026 showed that K. pneumoniae CICC 10011 is a potential strain for 2,3-BD production.

Key words: 3-butanediol, fermentation, metabolic flux analysis, metabolic characteristic

摘要： 对两株克雷伯氏菌(Klebsiella pneumoniae)批式流加发酵生产2,3-丁二醇进行了研究，结果表明，K. pneumoniae CICC 10011代谢产生的各种有机酸和乙醇浓度均明显低于K. pneumoniae DSM 2026，发酵56 h，目标产物(2,3-丁二醇+乙偶姻)浓度为85.61 g/L，生产强度为1.53 g/(L×h)，葡萄糖质量转化率为45%. 对2株克雷伯氏菌发酵的代谢流量分析表明，K. pneumoniae CICC 10011是生产2,3-丁二醇的优良菌株.

关键词: 3-丁二醇, 发酵, 代谢流分析, 代谢特性

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