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过程工程学报 ›› 2018, Vol. 18 ›› Issue (3): 632-638.DOI: 10.12034/j.issn.1009-606X.217355

• 生化工程 • 上一篇    下一篇

响应面法优化纤维素酶辅助从泡叶藻中提取泡叶藻聚糖的工艺

鲍青云1, 韦敬柳乙1, 姜泽东1,2,3,4*, 余 刚1, 黄高凌1,2,3,4, 朱艳冰1,2,3,4,肖安风1,2,3,4, 倪 辉1,2,3,4, 李清彪1,2,3,4   

  1. 1. 集美大学食品与生物工程学院,福建 厦门 361021;2. 福建省食品微生物与酶工程重点实验室,福建 厦门 361021; 3. 厦门南方海洋研究中心海藻资源化利用与深加工重点实验室,福建 厦门 361021;4. 厦门市食品与生物工程技术研究中心,福建 厦门 361021
  • 收稿日期:2017-10-10 修回日期:2017-11-23 出版日期:2018-06-22 发布日期:2018-06-06
  • 通讯作者: 姜泽东
  • 基金资助:
    国家自然科学基金项目;集美大学科研基金资助;福建省自然科学基金面上项目

Optimization of Cellulase Assisted Extraction Conditions for Sulfated Polysaccharide (Ascophyllan) from Ascophyllum nodosum by Response Surface Methodology

Qingyun BAO1,  Jingliuyi WEI1,  Zedong JIANG1,2,3,4 *,  Gang YU1,  Gaoling HUANG 1,2,3,4 Yanbing ZHU1,2,3,4,  Anfeng XIAO1,2,3,4,  Hui NI1,2,3,4,  Qingbiao LI1,2,3,4   

  1. 1. College of Food and Biology Engineering, Jimei University, Xiamen, Fujian 361021, China; 2. Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering Technology, Xiamen, Fujian 361021, China; 3. Key Laboratory of Systemic Utilization and In-depth Processing Economic Seaweed, Xiamen Southern Ocean Technology Center, Xiamen, Fujian 361021, China; 4. Research Center of Food and Biotechnology of Xiamen City, Xiamen, Fujian 361021, China
  • Received:2017-10-10 Revised:2017-11-23 Online:2018-06-22 Published:2018-06-06

摘要: 通过单因素实验研究纤维素酶辅助提取泡叶藻聚糖时液料比、酶添加量、酶解温度、酶解时间等关键因素对泡叶藻聚糖提取率的影响,并进一步采用Box-Behnken实验设计和响应面分析法优化其工艺参数. 结果表明,纤维素酶辅助提取泡叶藻聚糖的优化工艺条件为液料比30 mL/g、酶浓度200 IU/mL、酶解时间2.0 h、酶解温度50℃,该条件下多糖提取率为14.65%?0.73%,与模型预测值14.75%非常接近,采用响应面法对泡叶藻聚糖提取条件进行优化合理可行.

关键词: 纤维素酶, 辅助提取, 泡叶藻聚糖, 最佳工艺, 响应面法

Abstract: In order to obtain the optimum parameters for the cellulase assisted extraction of ascophyllan from brown seaweed Ascophyllum nodosum, the effects of several factors including water to biomass ratio, cellulase concentration, enzymolysis temperature, and enzymolysis time on the extraction yield of ascophyllan were investigated by using a single factor experiment and further optimized by using Box-Behnken experiment and response surface methodology (RSM). The results revealed that the optimal parameters for ascophyllan extraction were ratio of water to biomass 30 mL/g, cellulase concentration 200 IU/mL, enzymolysis temperature 50℃, enzymolysis time 2.0 h. Under these conditions, the actual extraction yield of ascophyllan was 14.65%±0.73%, in keeping approach with the predicted value by RSM (14.75%). Therefore, it is feasible that utilized RSM to optimize the conditions of extracting ascophyllan with the assist of cellulase.

Key words: cellulase, assisted extraction, ascophyllan, optimum technology, RSM