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›› 2014, Vol. 14 ›› Issue (4): 655-659.

• 生化工程专栏 • 上一篇    下一篇

钙离子对米根霉菌体形态及L-乳酸合成的影响

吴王杰 洪璐 孙小龙 付永前   

  1. 浙江台州学院生物质资源研究所 浙江台州学院生物质资源研究所 南京工业大学生物与制药工程学院 材料化学工程国家重点实验室 台州学院生命科学学院
  • 收稿日期:2014-06-12 修回日期:2014-07-21 出版日期:2014-08-20 发布日期:2014-08-20
  • 通讯作者: 吴王杰

Effects of Calcium Ion on the Morphology of Rhizopus oryzae and Production of L-Lactic Acid

WU Yu-jie HONG Lu SUN Xiao-long FU Yong-qian   

  1. Institute of Biomass Resources, Taizhou University Institute of Biomass Resources, Taizhou University College of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology College of Life Sciences, Taizhou University
  • Received:2014-06-12 Revised:2014-07-21 Online:2014-08-20 Published:2014-08-20
  • Contact: WU Yu-jie

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摘要： 采用添加钙离子的方法，考察钙离子对米根霉菌体形态及L-乳酸发酵合成的影响. 结果表明，添加钙离子有利于球状米根霉形成. 可溶性钙CaCl2浓度越低，越有利于形成较小直径的菌球，利于L-乳酸生成；添加较高浓度的难溶性钙CaCO3 (8.0 g/L除外)能形成较大菌球(约1.5 mm)，更有利于L-乳酸合成，产量达58.6 g/L，高于CaCl2的57.2 g/L，平均生产强度为1.22 g/(L×h)，高于CaCl2的1.02 g/(L×h). 但过量的CaCO3会包裹菌球，影响L-乳酸合成.

关键词: 米根霉, 形态, 钙离子, L-乳酸, 菌球, 发酵

Abstract: The effects of calcium ion on morphology of Rhizopus oryzae pellets during preculture and L-lactic acid production in fermentation were studied. The results showed that the addition of calcium ion could induce the pellet formation. The smaller pellets precultured with low concentration of soluble calcium (CaCl2) were beneficial to the production of L-lactic acid. By contrast, the larger pellets (1.5 mm) precultured with high concentration of insoluble calcium (CaCO3), except 8.0 g/L CaCO3, were beneficial to the production of L-lactic acid, the highest L-lactic acid productivities of 1.22 g/(L×h) and 58.6 g/L were achieved, which was better than those with CaCl2 at 1.02 g/(L×h) and 57.2 g/L. The excessive CaCO3 could envelope the pellets, and decreased its productivity when precultured with 8.0 g/L CaCO3.

Key words: Rhizopus oryzae, morphology, calcium ion, L-lactic acid, pellet, fermentation

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