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›› 2014, Vol. 14 ›› Issue (4): 660-663.

• 生化工程专栏 • 上一篇    下一篇

蔗糖与葡萄糖作辅助碳源对重组Klebsiella pneumoniae发酵生产 1,3-丙二醇的影响

吴家鑫 王凤寰 王峥 谭天伟 刘沛溢   

  1. 北京化工大学生命科学与技术学院，北京市生物加工重点实验室 北京化工大学生命科学与技术学院，北京市生物加工重点实验室 昆明理工大学冶金与能源工程学院 北京化工大学生命科学与化学技术学院 北京古龙嘉兰生物技术有限公司
  • 收稿日期:2014-06-27 修回日期:2014-07-18 出版日期:2014-08-20 发布日期:2014-08-20
  • 通讯作者: 吴家鑫

Effect of Sucrose and Glucose as Cosubstrate on 1,3-Propanediol Fermentation by Recombinant Klebsiella pneumoniae

WU Jia-xin WANG Feng-huan WANG Zheng TAN Tian-wei LIU Pei-yi   

  1. Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijiing University of Chemical Technology Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijiing University of Chemical Technology Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology College of Life Science and Technology, Beijing University of Chemical Technology Beijing Gulong Jialan Biotechnology Co.,Ltd
  • Received:2014-06-27 Revised:2014-07-18 Online:2014-08-20 Published:2014-08-20
  • Contact: WU Jia-xin

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被引次数

2

摘要： 实验研究了重组Klebsiella pneumoniae批式发酵生产1,3-丙二醇过程中辅助碳源蔗糖与葡萄糖对发酵过程的影响，对发酵工艺进行了放大，并对流加策略进行了优化. 结果表明，葡萄糖为发酵生产1,3-丙二醇的辅助碳源优于蔗糖；以重组Klebsiella pneumoniae为菌种，以葡萄糖为辅助碳源，采用指数流加策略，30 L发酵罐中1,3-丙二醇的产量最高达85.2 g/L，产率达0.63 mol/mol，比单纯以甘油为碳源分别提高37.35%和25.00%.

关键词: 3-丙二醇, 重组Klebsiella pneumoniae, 辅助碳源, 葡萄糖, 发酵, 指数流加

Abstract: The effects of glucose and sucrose as two kinds of cosubstrate on fed-batch fermentation of 1,3-propanediol by recombinant Klebsiella pneumoniae were investigated, and the scale-up of fermentation technology and optimization of feeding strategy also studied. The results showed that glucose as cosubstrate was better than sucrose. The final yield and productivity of 1,3-propanediol were 85.2 g/L and 0.63 mol/mol in 30 L fermentor by exponential feeding strategy with glucose, respectively. Compared with only glycerol as the carbon source, the yield and productivity of 1,3- propanediol were raised by 37.35% and 25.00%, respectively.

Key words: 3-propanediol, recombinant Klebsiella pneumoniae, cosubstrate, glucose, fermentation, exponential feeding

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