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›› 2009, Vol. 9 ›› Issue (6): 1164-1168.

• 生化工程专栏 • 上一篇    下一篇

超大孔聚苯乙烯微球固定蛋白质

曲瑞芳 孔英俊 曲建波 马润宇 苏志国 罗坚 罗坚   

  1. 北京化工大学生命科学与技术学院 中国科学院过程工程研究所生化工程国家重点实验室 中国科学院过程工程研究所生化工程国家重点实验室 北京化工大学化学工程学院 中国科学院过程工程研究所生化工程国家重点实验室 中国科学院过程工程研究所生化工程国家重点实验室
  • 收稿日期:2009-06-10 修回日期:2009-07-16 出版日期:2009-12-20 发布日期:2009-12-20
  • 通讯作者: 曲瑞芳

Separation and Immobilization of Protein on Macroporous Polystyrene Chromatography Microspheres

QU Rui-fang KONG Ying-jun QU Jian-bo MA Guang-hui, SU Zhi-guo SU Zhi-guo, LUO Jian,   

  1. College of Life Science and Technology, Beijing University of Chemical Technology Key Laboratory of Biochemical Engineering，Institute of Process Engineering，Chinese Academy of Sciences Key Laboratory of Biochemical Engineering，Institute of Process Engineering，Chinese Academy of Sciences School of Chemical Engineering, Beijing University of Chemical Technology State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences Key Laboratory of Biochemical Engineering，Institute of Process Engineering，Chinese Academy of Sciences
  • Received:2009-06-10 Revised:2009-07-16 Online:2009-12-20 Published:2009-12-20
  • Contact: QU Rui-fang

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摘要： 采用琼脂糖对孔径为300~500 nm的超大孔聚苯乙烯(MPS)微球进行亲水化修饰，并在修饰后的表面上偶联病毒蛋白颗粒(RV)，用于蛋白质的分离纯化实验. 结果表明，经过修饰的MPS层析介质具有优异的流体力学性能，层析过程的最高流速可达1120 cm/h，对病毒蛋白颗粒的固定量为0.638 mg/g，是商品化介质Sepharose 4FF的2倍. 采用RV-MPS介质对病毒蛋白颗粒抗体进行了分离纯化，收率为29.22%，高于Sepharose 4FF的18.92%.

关键词: 超大孔聚苯乙烯微球, 流体力学特性, 蛋白质, 分离, 固定

Abstract: Macroporous polystyrene microspheres with pore diameters between 300~500 nm were modified with hydrophilic agarose, and the hydrophilic surface was then immobilized with virus protein particles for protein purification experiments. The results showed that the modified macroporous polystyrene microspheres had excellent hydrodynamic characteristics, with a working flow rate as high as 1120 cm/h. The protein separation time was also greatly reduced compared with a commercially available agarose medium. The virus protein particle coupling capacity of 0.638 mg/g was about two times of commercially agarose medium. A recovery rate of 29.22% was achieved using RV-MPS medium, higher than 18.92% using commercially agarose medium.

Key words: macroporous polystyrene microspheres, hydrodynamic characteristics, protein, separation, immobilizatio

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