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过程工程学报 ›› 2015, Vol. 15 ›› Issue (4): 653-658.DOI: 10.12034/j.issn.1009-606X.215189

• 生化工程专栏 • 上一篇    下一篇

白油佐剂粒径均一性与炎症和免疫学效应的相关性

刘雁南 王连艳 杨婷媛 刘艳辉 马光辉   

  1. 北京化工大学生命科学与技术学院 中国科学院过程工程研究所生化工程国家重点实验室 中国科学院过程工程研究所生化工程国家重点实验室 北京化工大学生命科学与技术学院 中国科学院过程工程研究所生化工程国家重点实验室
  • 收稿日期:2015-04-16 修回日期:2015-05-15 出版日期:2015-08-20 发布日期:2015-08-20
  • 通讯作者: 刘雁南

Particle Size Uniformity of White Oil Adjuvant and Its Related Inflammation and Immunological Effect

LIU Yan-nan WANG Lian-yan, YANG Ting-yuan LIU Yan-hui MA Guang-hui   

  1. College of Life Science and Technology, Beijing University of Chemical Technology State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences College of Life Science and Technology, Beijing University of Chemical Technology State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences
  • Received:2015-04-16 Revised:2015-05-15 Online:2015-08-20 Published:2015-08-20
  • Contact: LIU Yan-nan

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1

摘要： 采用高速搅拌和快速膜乳化法制备鸡新流二联白油佐剂疫苗，考察了疫苗粒径均一性与疫苗引起的炎症反应和免疫学效应的关系. 结果表明，2种疫苗粒径约为1.0 mm，快速膜乳化疫苗(ME vaccine)的多分散系数(PDI)为0.036，高速搅拌疫苗(HS vaccine)的PDI值为0.279，前者粒径分布更均一. 两种疫苗的HI抗体和IgG抗体水平无显著差异. HS vaccine注射部位炎症因子IL-1b, IL-6, IL-17, TNF-a表达量最高分别为45, 7, 25和80 pg/mL，显著高于ME vaccine(均低于5 pg/mL). 均一白油佐剂疫苗在诱导有效免疫应答的同时，引起的炎症反应更轻.

关键词: 快速膜乳化, 均一性, 鸡新流疫苗, 炎症, 免疫

Abstract: Chicken NDV-AIV bigeminal vaccine white oil emulsion adjuvant was prepared by high speed stirring (HS) and rapid membrane emulsification (ME), respectively, the relationship between emulsion uniformity and inflammation and immunological effect injection sites was studied. The results showed that emulsion particle size of both kinds of vaccine were about 1.0 mm. Polydispesity index (PDI) value of ME vaccine was 0.036, and that of HS vaccine 0.279. ME vaccine showed better uniformity. The two kinds of vaccine had no significant difference in HI and IgG antibody level. Inflammatory factor IL-1b, IL-6, IL-17 and TNF-a gene expression levels of HS vaccine were up to 45, 7, 25 and 80 pg/mL, which were significantly higher than that of ME vaccine (the expression quantities were all less than 5 pg/mL). The white oil emulsion adjuvant with uniform size could induce enough immune protection, and cause milder inflammation.

Key words: membrane emulsification, uniformity, chicken NDV-AIV vaccine, inflammation, immunization

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