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›› 2014, Vol. 14 ›› Issue (1): 133-138.

• 生化工程专栏 • 上一篇    下一篇

重组肺炎克雷伯菌发酵联产3-羟基丙酸和1,3-丙二醇

李清 黄艳娜 李志敏 叶勤   

  1. 华东理工大学生物反应器工程国家重点实验室 华东理工大学生物反应器工程国家重点实验室 华东理工大学生物反应器工程国家重点实验室 华东理工大学海洋生化工程研究所 生物反应器工程国家重点实验室,
  • 收稿日期:2013-09-10 修回日期:2013-11-15 出版日期:2014-02-20 发布日期:2014-02-20
  • 通讯作者: 李志敏

Co-production of 3-Hydroxypropionie Acid and 1,3-Propanediol by a Recombinant Strain of Klebsiella pneumoniae

LI Qing HUANG Yan-na LI Zhi-min YE Qin   

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology
  • Received:2013-09-10 Revised:2013-11-15 Online:2014-02-20 Published:2014-02-20
  • Contact: LI Zhi-min

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摘要： 对表达了高效醛脱氢酶的重组肺炎克雷伯氏菌以甘油为底物生产3-羟基丙酸和1,3-丙二醇的过程进行优化，将发酵过程中补料阶段甘油浓度分别控制为0~10, 10~20, 20~30 g/L，并分3次间歇性补加甘油. 结果表明，发酵过程中补料阶段控制甘油浓度在20~30 g/L，发酵26 h得到47.20 g/L 3-羟基丙酸和43.90 g/L 1,3-丙二醇；而间歇性补加甘油产物得率最高，发酵26 h时3-羟基丙酸和1,3-丙二醇相对甘油的得率分别为0.35和0.38 mol/mol. 3-羟基丙酸和1,3-丙二醇联产可实现辅因子烟酰胺腺嘌呤二核苷酸的再生平衡，从而提高碳回收率.

关键词: 肺炎克雷伯氏菌, 3-羟基丙酸, 3-丙二醇, 辅因子再生, 发酵, 优化

Abstract: The production of 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO) by recombinant Klebsiella pneumoniae over-expressed aldehyde dehydrogenase was optimized with glycerol as substrate. During the feeding stage of fermentation, glycerol concentration was controlled differently at 0~10, 10~20, 20~30 g/L and intermittent addition of glycerol for three times. The results showed that the process controlling of glycerol concentration at 20~30 g/L resulted in the highest titer, 47.20 g/L of 3-HP and 43.90 g/L of 1,3-PDO were obtained in 26 h. The intermittent addition of glycerol led to the highest yield of 3-HP and 1,3-PDO, 0.35 and 0.38 mol/mol, respectively. The co-production of 3-HP and 1,3-PDO could achieve cofactor regeneration of NAD+ and NADH balance, which enhanced the recovery of carbon.

Key words: Klebsiella pneumoniae, 3-hydroxypropionic acid, 3-propanediol, co-factor regeneration, fermentation, optimization

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