关键词: 二苯并噻酚, 生长, 生物脱硫, 德氏假单胞菌

Abstract: Pseudomonas delafieldii R-8 isolated from coal contaminated soils in our laboratory could metabolize DBT to 2-hydroxybiphenyl via the sulfur-specific pathway. Effects of initial pH, sources of carbon and nitrogen of the culture media were investigated by using DBT as the sole source of sulfur in aqueous phase. It was indicated that this strain grew well in the pH range of 6~9 and the optimal temperature for growth was 30oC. Glycerol was the optimal carbon source and the optimal concentration was 10 g/L. Organic nitrogen sources including yeast extract, bacto-typtone and beef typtone were conducive to the growth of this strain, but repressed the expression of the desulfurization activity of R-8 even in the presence of DBT. Ammonia acetate was the appropriate source of nitrogen at the concentration of 1.6 g/L. Although sodium sulfate, dimethyl sulfoxide, methionine, cysteine and DBT could be used as the sole sources of sulfur respectively, these sulfur-containing compounds except DBT could not induce the expression of the biodegradation activity of R-8.

Key words: biodesulfurization, dibenzothiophene, growth, Pseudomonas delafieldii