Preparation of Thymalfasin Loaded PLGA Microspheres by Premix Membrane Emulsification as Long-term Effective Formulation
YANG Liu-qing WANG Li-qiu WU Jie QI Feng REN Yu TAO An-jin MA Ya-ping MA Guang-hui
Environmental and Chemical Engineering College , Yan Shan University Environmental and Chemical Engineering College , Yan Shan University National Engineering Research Center for Biotechnology State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences HanYu Pharmaceutical Enterprise co. HanYu Pharmaceutical Enterprise co. State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences
Abstract：Thymalfasin-loaded biodegradable poly(lactic-co-glycolic acid) (PLGA) microspheres were prepared by premix membrane emulsification technique combined with solvent evaporation method. The effects of concentrations of PLGA and Arlacel 83 in oil phase, pH value of external water phase and internal water volume on the drug encapsulation efficiency and size distribution of microcapsules were examined. The results showed that the optimized preparation conditions of thymalfasin loaded PLGA microspheres were molecular weight of PLGA 51 kDa, concentrations of PLGA and Arlacel 83 in oil phase 100 and 10 g/L, inner water volume 0.5 mL, and pH value of outer water phase 3.5. The microspheres prepared under the optimal conditions had narrow size distribution (Span<0.7), high drug encapsulation efficiency (above 80%), low initial burst release (below 20% in initial 24 h) and constant release rate during 30 d.
2014, Vol. 14 
