关键词: 葡萄藻(Botryococcus braunii), 生长, 无机碳源, 氮源, 磷源, NaF

Abstract: Extracting penicillin by aliphatic alcohols was studied in this work. The aliphatic alcohols were more efficient than butyl acetate in extracting penicillin at pH=4. Mixtures of aliphatic alcohol and butyl acetate showed strong coordinated extraction effect. Aliphatic alcohols with branched chain had a little stronger ability to extracting penicillin than straight chain alcohols with the same number of carbon atoms, and water content in organic phase was reduced at the same time. Results of FT–IR indicated, that with adding of butyl acetate, hydroxyl group spectra of aliphatic alcohol became wider and shifted to higher wavenumber. It suggested that butyl acetate molecular blocked forming of aliphatic alcohol congeries, which made hydroxyl group more active. It was suggested by FT-Raman that penicillin molecule entered alcohol phase in inverse microemulsion. The branched chain of alcohols made the system more orderly and polar, which was also favorable to extraction of penicillin.

Key words: Botryococcus braunii, growth, incarbon sources, nitrate, phosphate, NaF