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›› 2002, Vol. 2 ›› Issue (4): 0-0.

• 4 •    

包裹定向排列纳米氯化银的二氧化硅纳米纤维

李永军,刘春艳,张志颖   

  1. 中国科学院理化技术研究所, 北京 100101
  • 出版日期:2002-08-20 发布日期:2002-08-20

Fed-batch Cultivation of Botryococcus braunii in an Air-lift Photobioreactor

WANG Jun, YANG Su-ling, CONG Wei, CAI Zhao-ling   

  1. 1. State Key Lab. Biochem. Eng., Inst. Chem. Metall., Chinese Academy of Sciences, Beijing 100080, China; 2. College of Life Science, Hubei University, Wuhan, Hubei 430062, China
  • Online:2002-08-20 Published:2002-08-20

摘要: 介绍了一种通过静电作用合成AgCl–SiO2复合材料的新方法. 这种方法能够制备出线条均匀的纳米二氧化硅纤维,直径范围在30~100 nm,纤维的长度大于20 mm. 所包裹的AgCl纳米粒子在纤维中呈规则排列. 沿着二氧化硅纤维生长方向,同一条二氧化硅纤维包裹多条氯化银纳米线.

关键词: 氯化银, 二氧化硅, 纳米粒子, 纳米纤维, 静电自组装

Abstract: Bacteriocin nisin was isolated and purified in temperature-induced aqueous two-phase systems using the capacity of extracting hydrophobic proteins of TritonX-114. The concentrations of sodium chloride and TritonX-114 which can form the aqueous two-phase systems were optimized by the response surface method. The full-factorial design was used to assess the main effects of factors and determine the path of steepest ascent to the optimal region of the aqueous two-phase system composition. The optimum concentrations of TritonX-114 and sodium chloride were then easily determined by using a central composite design and were found to be 3.27% of TritonX-114 and 0.85 mol/L sodium chloride. The recovery and purification factor were 92% and 9.05 respectively under the optimum conditions.

Key words: Botryococcus braunii, air-lift photobioreactor, nitrate, phosphate, fed-batch

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