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›› 2007, Vol. 7 ›› Issue (4): 782-785.

• 生化工程专栏 • 上一篇    下一篇

光质对玛咖愈伤组织生长、分化的影响

王亚丽,王晓东,赵兵,王玉春   

  1. 中国科学院过程工程研究所生化工程国家重点实验室
  • 出版日期:2007-08-20 发布日期:2007-08-20

Effects of Light Quality on Maca Callus Growth and Differentiation

WANG Ya-li,WANG Xiao-dong,ZHAO Bing,WANG Yu-chun   

  1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering
  • Online:2007-08-20 Published:2007-08-20

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摘要： 研究了在组织培养过程中光质对玛咖愈伤组织生长、分化及细胞内糖代谢关键酶的影响. 在红光、黄光和白光下培养25 d, 玛咖愈伤组织的干重明显高于在绿光和蓝光下. 在分化培养基上，红光、黄光和白光条件下玛咖愈伤组织的出芽率达78%~82%，而在绿光和蓝光下出芽率几乎为0. 进一步研究表明，红光、黄光和白光条件下糖代谢中3种关键酶(葡萄糖-6-磷酸酶、己糖激酶、苹果酸脱氢酶)的活性明显高于绿光和蓝光条件下，与愈伤组织生长、分化情况相关联，说明细胞内的糖代谢受光质调节并参与了对愈伤组织生长和分化的调节.

关键词: 玛咖, 光质, 生长, 分化, 糖代谢

Abstract: The effects of light quality on maca (Lepidium meyenii) callus growth, differentiation and the key enzymes in glycolysis were investigated. Cultured for 25 d under red light, yellow light and white light, the dry weights of maca callus were heavier than those under green light and blue light. The induction rates of maca callus cultured on the differentiation culture media under red light, yellow light and white light were 78%~82%. However the induction rate was zero under green light and blue light. The activities of three key enzymes in glycolysis such as glucose-6-phosphate dehydrogenase (G-6-PDH), hexokinase (HK) and malate dehydrogenase (MDH) under red light, yellow light and white light were significantly higher than those under green light and blue light. The changes of enzyme activity were associated with the states of growth and differentiation of maca callus. The results showed that the glycolysis in maca callus was regulated by light quality and took part in regulating callus growth and differentiation.

Key words: maca, light quality, growth, differentiation, glycolysis